By Cheryl L. Scudamore
A useful consultant to the Histology of the Mouse provides a full-colour atlas of mouse histology. Mouse types of illness are used largely in biomedical examine with many hundreds of thousands of recent versions being generated every year. entire phenotypic research of all of those versions can make the most of histologic overview of the tissues.
This e-book is aimed toward veterinary and scientific pathologists who're strange with mouse tissues and scientists who desire to overview their very own mouse types. It presents functional assistance at the assortment, sampling and research of mouse tissue samples so that it will maximize the knowledge that may be received from those tissues. in addition to illustrating the conventional microscopic anatomy of the mouse, the publication additionally describes and explains the typical anatomic diversifications, artefacts linked to tissue assortment and history lesions to assist the scientist to tell apart those adjustments from experimentally- prompted lesions.
This may be a necessary bench-side significant other for researchers and practitioners searching for an obtainable and well-illustrated consultant to mouse pathology.
- Written via skilled pathologists and particularly adapted to the wishes of scientists and histologists
- Full color throughout
- Provides suggestion on sampling tissues, necropsy and recording data
- Includes universal anatomic diversifications, historical past lesions and artefacts to be able to aid non-experts comprehend even if histologic adaptations noticeable are a part of the conventional heritage or relating to their experimental manipulation
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Extra resources for A Practical Guide to the Histology of the Mouse
Treuting and S. Dintzis), Elsevier, Amsterdam, pp. 15–41. E. et al. (2002) Recommended approaches for the evaluation of testicular and epididymal toxicity. Toxicologic Pathology 30, 507–520. , Jonassen, H. M. (2002) Fixation of testes and eyes using a modiﬁed Davidson’s ﬂuid: comparison with Bouin’s ﬂuid and conventional Davidson’s ﬂuid. Toxicologic Pathology 30, 524–533. J. (1984) Spontaneous lesions of the mouse biliary tract. Journal of Comparative Pathology 94, 263–271. J. (1969) The so-called Swiss mouse.
Published guides for sampling and trimming most mouse tissues are available (Ruehl-Fehlert et al. 2003; Kittel et al. 2004; Morawietz et al. 1). A tissue trimming guide should include: • • • • tissue to be sampled; site of sampling – may specify region, such as kidney cortex or liver lobe; number of samples – for example, single section, multiple sections from different lobes/areas; plane of sampling – cross section or longitudinal; • • • • 27 size of sample; orientation of tissue in block – for example, which tissue or region is closest to slide label or which face of the tissue (up or down) is sectioned of the tissue; whether left or right samples should be identiﬁed separately – for example, unilateral lesions; separation of tissues that look the same when ﬁxed/processed – for example, identiﬁcation of the individual lymph nodes.
Et al. (2007) Evaluation of organ weights for rodent and non-rodent toxicity studies: 23 a review of regulatory guidelines and a survey of current practices. Toxicologic Pathology 35, 742–750. Moolenbeek, C. J. (1981) The ‘Swiss roll’: a simple technique for histological studies of the rodent intestine. Lab Animal 15, 57–59. , Ruehl-Fehlert, C. and Kittel, B. et al. (2004) Revised guides for organ sampling and trimming in rats and mice – Part 3. A joint publication of the RITA and NACAD groups.
A Practical Guide to the Histology of the Mouse by Cheryl L. Scudamore